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Monday, August 12, 2019

The rapid chromatographic method is a technology widely used in the separation and purification of active target compounds, such as organic compounds, natural products, and biological macromolecules. Rapid chromatographic method has the advantages of easy-operation, low-price, and fast-analysis...

Monday, August 12, 2019

The system of an HPLC column is composed of a liquid, a reservoir, a pump, a sampler, a column, a detector and a recorder. - The packing material of HPLC columns - The packing material has high carbon content, small surface polarity, very low chemical purity and high surface separation...

Friday, August 09, 2019

- Some Tips In QuEChERS D-SPE? - Why choose anhydrous magnesium sulfate as a water remover? In the d-SPE purification process, the moisture content plays a crucial role in the purification effect of PSA. The lower the moisture content, the better the purification effect of PSA. Experiments...

Friday, August 09, 2019

Reversed-phase chromatography (RPC) contains any chromatographic method using a non-polar stationary phase. The word “reverse” has a historical background. In the 1970s, most liquid chromatography was performed on unmodified silica or alumina. Their surface chemistry is hydrophilic...

Thursday, August 08, 2019

More than 90% of gas chromatographic problems occur in the inlet, and it is necessary to see which problems may occur in the inlet. Problem No.1: Leak If there is a leak, the pressure on the inlet may not be maintained, and the instrument will alarm. If the problem cannot be solved in time...

Thursday, August 08, 2019

Flash columns adopt high-capacity silica gel for precise filling, which has very excellent separation and purification performance. The specific surface area of high-capacity spherical silica gel is 40% higher than that of ordinary spherical silica gel, and the sample loading is twice...

Thursday, August 08, 2019

Chromatography is a separation and analysis method and the separation is the core. Therefore, HPLC columns are the heart of the chromatographic system. The requirements for the column are high efficiency, good selectivity and fast analysis speed. Generally, the column length of about...

Tuesday, August 06, 2019

In recent years, the application of HPLC in inorganic analysis has developed rapidly, and the use of flash columns has been more and more widely because of a method that can rapidly determine heavy metal elements in environmental water samples. Matters needing the attention of flash columns 1...

Tuesday, August 06, 2019

HPLC is more suitable for the separation and analysis of substances with the high boiling point, poor thermal stability, physiological activity, and relatively large molecular weight. Hawach HPLC columns are widely used in the analysis of nucleic acid, peptide, lactones, polycyclic...

Monday, August 05, 2019

One of the main factors affecting the life of the liquid chromatographic column is the mobile phase. There are three aspects of the mobile phase factors. The pH value of the Mobile Phase The fillers of different substrates apply to different pH value range. Under normal temperature...

Friday, August 02, 2019

Flash column pressure has something to do with the form of silica gel, the mesh number, the synthesis condition of filler, the condition of column packing, the mobile phase, and the temperature when analyzing. Different manufacturers' new flash columns, their column pressures may...

Friday, August 02, 2019

Usually, the sample contains something that is not of interest to the analyst. Salt, lipids, lipid-containing substances, humic acid, hydrophobic proteins, and other biological substances are some substances that may interact with the HPLC column in use. These substances have less...

Thursday, August 01, 2019

There are two different cases of retention time: retention time drift and retention time fluctuations. The former means that the retention time changes only in one direction, while the latter refers to the fluctuation of the retention time without a fixed law. It is often helpful...

Thursday, August 01, 2019

It is important for the experimenters to use and maintain chromatographic columns correctly. They should focus on many some things so as to protect the columns in the using process. Here we talk about several attentions. Avoid changing pressure and temperature dramatically and any...

Wednesday, July 31, 2019

- The Selection Principle of Silica Gel Column - Commonly, the maximum sample loading volume of a silica gel column is about 1/20 of the volume of the column's silica gel. However, as a practical matter, specific sample loading volume should be decided by each constituent's degree...

Tuesday, July 30, 2019

The improper use of chromatographic columns will make the columns decrease or lose effectiveness. Generally speaking, the columns always have problems at the end of them. If we can install a short column with the same stationary phase as the analysis column in front of analysis column...

Friday, July 19, 2019

Bonded-phase chromatography is a liquid-liquid chromatography method in which a stationary phase is covalently bound to a carrier particle, which overcomes the mechanical shock in the distribution chromatography, the constant loss of the stationary phase and the gradual change of...

Monday, July 15, 2019

Laboratory data show that the optimum flow rate of silica gel flash columns is decided by the length and width of columns and the property of gel. To be more specific, longer and narrower columns will provide more theoretical plates which are able to affect the flow rate. When referring...

Monday, July 15, 2019

Laboratory workers use a membrane to filter two phases. Actually, the membrane is a kind of media. According to different aperture sizes, filter membranes can be divided into the microporous membrane, ultrafiltration membrane, nanofiltration membrane a nd reverse osmosis membranes...

Monday, July 15, 2019

All the technique cores of HPLC from Hawach Scientific are focused on the characteristics of the chromatogram column. Quality Recognition This is shown as the fact that terminal customers have published more than 500 papers on those journals at home and abroad. For famous oversea journals...

Monday, July 15, 2019

The solid phase extraction cartridges (SPE column, or Solid Phase Extraction Cartridges, or SPE cartridges for short) is a sample pretreatment device for extraction, separation and concentration developed from a chromatography column. Mainly used in sample pretreatment of various foods...

Friday, July 12, 2019

Today, Hawach want to talk about the pre-treatment process for pesticide residue detection from the perspective of food safety – QuEChERS, and also tell you how to pre-treat pesticide residue food. Introduction The present analytical methods and determining techniques, pre-treatment...

Thursday, July 11, 2019

The formation of a flash phenomenon The boiling point of water is higher than 100℃ when the water is heated under a certain pressure. The higher the pressure, the higher the boiling point of water and the higher the heat content. When the pressure decreases, some sensible heat is released...

Thursday, July 11, 2019

The formation of a flash phenomenon The boiling point of water is higher than 100℃ when the water is heated under a certain pressure. The higher the pressure, the higher the boiling point of water and the higher the heat content. When the pressure decreases, some sensible heat is released...

Thursday, July 11, 2019

Columns Ahead Statement Generally speaking, SPE/FLASH is mainly applied to the pretreatment of samples. People can easily perceive that SPE/FLASH columns separate salt and sugar, while HPLC ones can make glucose, maltose and sucrose apart from the sugar solution, which posses more...

Thursday, July 11, 2019

With the development of society and technology, chromatographic analysis theory has also been developing rapidly. HPLC column has now been widely applied in every field of laboratory analysis. HPLC column gathers function of both detection and separation. With the right choice, use...

Thursday, July 11, 2019

With the development of society and technology, chromatographic analysis theory has also been developing rapidly. HPLC column has now been widely applied in every field of laboratory analysis. HPLC column gathers function of both detection and separation. With the right choice, use...

Thursday, July 11, 2019

SPE column or SPE cartridges are based on liquid-solid phase chromatography theory, it is a kind of extraction, separation, concentrated sample preparation unit. Mainly used in sample pretreatment of various foods, agricultural and livestock products, environmental samples and target...

Tuesday, July 09, 2019

Rapid chromatography Technology for the separation and purification of natural products and biological macromolecules. It has the advantages of easy operation, cheap price, and rapid analysis, and a few other technologies can match the rapid chromatography in the application of purifying...

Tuesday, July 09, 2019

The reversed-phase HPLC columns may be repeatedly injected into a sample containing a strongly retained material, and in particular, the sorbent components such as large molecular weight or hydrophobic biological flow proteins and strong alkaline substances may be adsorbed on the silica medium...