The Choice, Use, and Maintenance of HPLC Column
With the development of society and technology, chromatographic analysis theory has also been developing rapidly. HPLC column has now been widely applied in every field of laboratory analysis. HPLC column gathers function of both detection and separation. With the right choice, use, and maintenance, you can keep good performance of the HPLC column. Otherwise, you will make it scrap fastly.
HPLC is a branch of chromatographic, using high-pressure infusion system to make the single or mixed solution and buffer mobile phase pump into the column with the stationary phase. After each component is separated, the solution can flow into the detector in order to be analyzed. Normally, the great diversity and complex matrix of the sample are key reasons to make HPLC column be easily polluted.
The Choice of HPLC Column
In general, the first thing you have to consider when choosing an HPLC column is the sample's cleanliness. For most researchers, the most appropriate length of guard column is 2 cm or 3 cm. The length of a guard column, the more chromatographic packings can be filled. Meanwhile, longer guard column can reduce more pollutants and extend the retention time of samples.
The Right Use of HPLC Column
1. Add Guard Column
2. Filter Mobile Phase and Sample
3. Purify Sample
4. Avoid Overhigh Column Pressure
5. Pay Attention to Temperature and PH Level
6. Correct and Timely Wash
The maintenance of the HPLC Column
1. Softly and Moderately Change and Handle the Column
2. Add One Column for Each Analytical Instrument to Extend Service Life
3. Avoid Sudden Change of Temperature and Pressure Causing the Mechanical Shock
4. Generally, Do Not Recoil HPLC Column
5. Do Not Directly Infuse Samples with Complex Matrix into HPLC Column
6. Fill HPLC Column with Acetonitrile or Methanol to Preserve It
7. Wash the Chromatographic Analysis System Timely after Use
Contact : Bruce Lu, +86 29 89284429
Good deal: buying from seller
We invite you to read our terms of use. You can also visit our FAQ section and see our information section on the risks associated with counterfeiting.
This page is about importers and exporters of The Choice, Use, and Maintenance of HPLC Column Search in a category : Everything else / Bulk Search in a category : choice, column, hplc, maintenance |
Wednesday, September 14, 2016
Quantity : 5000 - Price : 50,00 €
Long life-Heavy Duty Bellows Seal Stop Valves DIN Standard Long Life-Heavy Duty Bellows Sealed Stop Valves DIN Long life bellows Seal Stop Valve DIN Long life bellows globe valve DIN Long bonnet bellows globe valve DIN Faltenbalgventil lange Bauform DIN Válvulas de fuelle de...
ETM Armaturen
- 59590 - Henri Durre
- 86 577 67953935
Thursday, December 12, 2024
▲VE-30RFM RX (Front / Rear) ▲VE-30RFM TX (Front / Rear) VE-30RFM HDMI to RF Matrix Extender is designed to convert HDMI signal to HD digital TV signal based on DVB-T CATV signal, it uses 64QAM mode to realize the HD digital TV signal conversion and transmit via coaxial cables for...
Meicheng Audio Video Co., Ltd.
- meicheng
- 23511 - New Taipei City
- 886 2 82280311
Sunday, May 29, 2016
Quantity : illumité - Price : négociable
Hello Ms. / Mr, Thank you for your attention. We are now Dray, based in Tunisia, Sfax precisely the economic capital of the country. Dray is a company that is developing in various sectors such as industry and trade of shoes ready to wear trade, general trade of all finished and semi...