Principles And Specifications Of Flash Chromatography Columns
As a unique chromatography technique that pushes solvent through the column uses by compressed gas or a pump, flash column chromatography is highly appreciated as an advance technique because it makes flow rates of the solvent much faster than the gravity flow.
Developed in the 1970's, flash column chromatography was upgraded from long column chromatography technology in the lab. At that time, the scientists were tired of long column chromatography, because it's kind of time-wasting with poor results. But flash column chromatography can not only provide better separation but also reduce the running time of the sample separations. The whole process usually takes about 5-10 minutes.
The silica gel is not only the gel which was first used in the column, but it is widely employed still nowadays. The air was pressured to drive the solvent through the silica gel column and compress it. After the sample is applied, and the same solvent is used to pass the sample through the column. The solvent can be different sometimes. And then, it's the time we can collect the purified component, small fractions emerge first, and the larger ones go after.
Principles And Specifications Of Flash Chromatography Columns
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