Preparative Liquid Chromatography System

Hanbon preparative liquid chromatography system has the characteristics of high efficiency, high speed, high automation, and explosion-proof, and is widely used in the separation and purification of pharmaceutical intermediates, biopharmaceuticals, synthetic drugs, natural products, fine chemicals, food additives and other fields. Including: CS-Prep® industrial preparative adsorption hplc system, Newstyle® laboratory liquid chromatography system, Intepure™ Integrated liquid chromatograph etc.

The Difference Between Analytical And Preparative Hplc System

Analytical chromatography, reflecting the composition of the sample, seeks efficient separation and high sensitivity without the need to collect specific fractions, and eluates are usually disposed of as waste liquids. In preparative process chromatography, however, the maximum amount of product must be produced in the minimum amount of time in order to save investment and operating costs. Due to the difference in purpose, there are major differences in the optimisation of the operating parameters chosen for preparative chromatography and analytical chromatography, including column size, packing, flow rate, operating pressure, injection volume, product purity, product recovery and chromatographic separation effectiveness. The former requires the smaller the injection volume, while the latter requires the column to be overloaded in order to increase the preparation volume. There are two types of column overloading, one is "mass overloading", where a smaller injection volume is maintained and the injection concentration is increased, and the other is "volume overloading", where a smaller injection concentration is maintained and the injection volume is increased. In preparative chromatography, mass overloading is generally considered to be preferable. In preparative chromatography separations, both separation and column efficiency decrease as the degree of mass overloading increases. However, operating with mass overload can increase the preparative volume provided that the separation can be achieved.

Preferred Options For Impurity Preparation

Impurity studies are carried out throughout the life cycle of pharmaceutical development. Impurity preparation is one of the bitter seas of sample purification, and the traditional approach is to use glass chromatography columns or analytical or semi-preparative HPLC to enrich bit by bit. The DAC50 with NS4210 chromatography system is 5-20 times more efficient than a 20 ml/min system with high throughput screening + excellent batch reproducibility. The system can also be equipped with an optional automatic fraction collector to increase automation and allow more focus on drug development.

For more information about hplc chromatography steps and latest hplc instruments, please feel free to contact us!