Keratinase
Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.Specification of Keratinase Enzyme
Name Keratinase Product Series Name NutriGen Keratinase
Activity 100000-200000 U/g
Strain Parengyodontium album EC code EC 3.4.99.11
Form Powder,Liquid Carrier Starch
Color White, Light yellow Loss on drying ≤ 10%
Working pH 6.0-12.5 Optimum pH 11
Working T 30℃-70℃ Optimum T 60℃
Particle size 80% pass 40 mesh
Package 25kg /bag
Storage Stored 6 months in dark, cool and dry conditions
Caution Avoid direct sunlight, high temperature
Definition and Dosage of Activity Keratinase
One unit (U) of protease activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.
Recommended dosage is 50-100g/t with 50000 U/g in complete feed.
Functions of Keratinase
1. Supplement the deficiency of endogenous protease for animal.
2. Increase the digestibility of protein and amino acid in poultry and swine feed ingredients.
3. Degrade the trysin inhibitor and lectin, this is what endogenous protease can't do.
4. Optimized the use of lower digestibility feed ingredient e.g corn, sorghum, feather meal,soybean meal and blood plasma powder.
5. Creat a better environment for animal and decrease pollution from nitrogen for human.
Application of Keratinase
1. The matrix value of keratinase (100g/t)
Nutrient Poultry
Value Amount provided in the diet
Metabolizable energy(Mcal/kg) 600 0.06
Crude protein, % 5400 0.54
Lysine, % 150 0.015
Methionine, % 180 0.018
Cystine, % 150 0.015
Threonine, % 210 0.021
Tryptophane, % 90 0.009
Isoleucine , % 150 0.015
Arginine, % 285 0.0285
Valine, % 255 0.0255
2. Application case: Improve the performance and digestibility of nutrients.
Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.
Experimental design:
Table1. Experimental design
Item Diet
PC Basal diet
NC Basal diet-0.5% crude protein
Group I NC+125g/t competitor
Group II NC+100g/t Keratinase(30000U/g)
Experimental cycle: 42 days
Results:
Table2 Effect of each treatment on the growth performance of AA broilers (g, %)
Items PC NC Group I Group II
Initial weight,g 62.79±0.31 62.58±0.29 62.56±0.33 62.33±0.27
1-21days
21stAG,g 986.16ab±10.08 977.41b±8.22 1013.59ab±25.72 997.17ab±10.53
ADFI,g 62.08± 1.43 60.93±1.10 62.09±2.04 58.71±2.12
ADG,g 43.94±0.47 43.45±0.39 45.12±1.29 44.33±0.53
F/G 1.41±0.03 1.40±0.03 1.38±0.05 1.33±0.04
Death rate,% 1.43±1.57 3.45±2.21 5.38±6.24 3.33±5.24
22-42days
22ndAG,g 1140.08±1.30 1135.33±5.97 1136.73±7.06 1138.33±5.41
42ndAG,g 2985.77±41.56 2971.06±47.61 3058.21±58.29 3010.25±44.17
ADFI,g 179.00±3.12 178.64±2.86 175.81±3.64 174.22±2.64
ADG,g 87.88±2.01 87.42±2.43 91.50±2.85 89.14±2.20
F/G 2.04±0.07 2.05±0.03 1.93±0.05 1.96±0.06
Death rate,% 2.00±2.19 1.33±2.07 0.67±1.63 1.33±2.07
1-42days
ADFI,g 120.51±2.39 119.78±1.63 118.94±2.01 116.47±1.60
ADG,g 69.59±0.98 69.25±1.13 71.32±1.39 70.18±1.05
F/G 1.73±0.02 1.73±0.02 1.67±0.02 1.66±0.03
Death rate,% 3.43±3.76 4.78±1.73 6.05±5.74 4.67±4.59
Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).</span>
Diagram1 Effect of keratinase on the nutrient digestibility of broilers
Table3 Effect of keratinase on the amino acid digestibility of broilers (%)
Items PC NC Group I Group II
Asp 55.87±4.44 52.59±2.57 54.52±8.03 57.52±5.76
Thr 63.09±7.47 61.47±5.32 62.17±5.74 63.69±5.19
Ser 69.11±5.51 65.44±4.61 65.80±7.04 68.04±4.10
Glu 69.31±3.41ab 64.68±1.35b 67.12±5.82 74.82±3.39a
Gly 55.36±15.14ab 42.03±4.21b 49.11±11.59b 67.00±1.89a
Ala 31.82±2.11b 34.51±4.37b 35.35±5.19 35.92±4.82b
Val 46.32±0.75 45.59±1.66 44.68±4.68 50.18±3.73
Met 72.53±1.35 70.69±2.65 75.17±3.79 74.29±3.31
Ile 46.45±0.53b 47.40±2.87ab 47.47±5.60 51.65±4.65ab
Leu 56.28±1.57 52.87±7.19 55.44±8.01 53.05±4.90
Tyr 50.93±3.67 47.91±4.34 50.94±10.97 56.55±8.06
Phe 66.11±2.37 61.58±1.25 62.81±7.79 68.80±6.93
His 31.90±4.72ab 25.09±7.33b 28.06±14.13 40.79±5.43ab
Lys 78.58±4.31ab 71.44±2.54b 74.47±8.35 82.45±1.20a
Arg 79.15±2.86ab 75.18±3.10b 75.80±5.99 80.68±3.36ab
Pro 67.76±0.73b 70.57±1.32ab 69.36±7.39 68.06±2.90b
TAA 62.69±0.43ab 59.17±1.39b 60.83±5.69 66.45±3.73a
Conclusion of Keratinase
On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.
Now the price of enzyme china is reasonable, if you have needs, please contact us.
Contact : Xi Julia, 86 531 55772899
Good deal: buying from seller
We invite you to read our terms of use. You can also visit our FAQ section and see our information section on the risks associated with counterfeiting.
This page is about importers and exporters of Keratinase Search in a category : Child clothing Search in a category : keratinase |
Friday, June 10, 2022
Beta Mannanase Enzyme β mannanase is a feed hemicellulase fermented by the improved strains using a liquid fermentation technique, and produced by an advanced post-treatment technique. It has properties of high activity, high quality, and good heat-resistant. Specification of Beta...
Jinan Bestzyme Bio-Engineering Co., Ltd
- 250002 - Jinan
- 86 531 55772899
Friday, April 28, 2023
Quantity : 5000T - Price : $1-$50/KG
Alpha galactosidase is a feed hemicellulose produced by a liquid submerged fermentation and advanced post-treatment technique. Alpha galactosidase can degrade galactosides, such as stachyose and raffinose, which can be fermented by microorganism in gut and cause flatulence in the intestine...
Jinan Bestzyme Bio-Engineering Co., Ltd
- 250002 - Jinan
- 86 531 55772899
Tuesday, August 02, 2022
Cellulase products is extracted from choiceness strains by microbial fermentation technology, and advanced post-processing techniques. As a kind of multi-component compound enzyme, cellulase contains several kinds of enzyme with synergistic effect which can catalyze the hydrolysis...
Jinan Bestzyme Bio-Engineering Co., Ltd
- 250002 - Jinan
- 86 531 55772899