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Introduction To Flash Chromatography

Flash chromatography is one of the methods in the preparation of liquid chromatography and is usually used for the separation of organic compounds. Flash chromatography has the advantages of ease of operation, low cost, and fast analysis. There are few other techniques for purifying organic compound applications that are comparable to flash chromatography.

Flash chromatography has become a versatile method for normal phase separation by purification. Flash chromatography is a typical low-pressure technique, and scientists are using the vacuum or pump technology to accelerate the separation of flash chromatography at medium pressure. The flash column is filled with a silica gel adsorbent with a particle size of 40-60 mm. A low viscosity mobile phase requires a smaller particle size. Traditional flash chromatography requires scientists to fill the flash column as needed for testing, so many columns become disposable prefabricated columns.

Flash chromatography is often used to scale up to normal phase chemicals separated by thin-layer chromatography. The demand for flash chromatography comes mainly from the pharmaceutical industry, biotechnology, and academic institutions, which account for 84% of the market for fast chromatography. In the pharmaceutical industry, flash chromatography is used in a wide range of applications, including the purification of small amounts of compounds, peptides, and purification of natural products.

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