Factors Affecting the Performance of C18 HPLC Columns
Physical properties of the chromatographic column could affect the performance of C18 HPLC columns.
Performance factors of C18 HPLC columns:
Silica gel purity
Class A silica gel has high residual silica hydroxyl with a negative charge and high metal content on the acidic surface, which leads to trailing of alkaline compounds.
Due to the low metal content and high silica hydroxyl pKa of class B silica gel, tail dragging of alkaline compounds does not occur.
Performance factors of C18 HPLC columns:
Column size
Short column – short running time, low column pressure
Long column – high resolution, long running time
Narrow diameter column – detector has high sensitivity
Performance factors of C18 HPLC columns:
Particle shape and size
When the mobile phase with high viscosity is used, the spherical particles can reduce the column pressure and prolong the life of the column. Smaller particles have higher column efficiency but can cause column pressure to be too high.
Performance factors of C18 HPLC columns:
Surface area and aperture
The high surface area has strong retention capacity, column capacity and separation degree for separating multi-component samples. Low surface area fillers usually reach equilibrium quickly and are particularly important for gradient leaching.
Contact : Bruce Lu, +86 29 89284429
Good deal: buying from seller
We invite you to read our terms of use. You can also visit our FAQ section and see our information section on the risks associated with counterfeiting.
|
This page is about importers and exporters of Factors Affecting the Performance of C18 HPLC Columns Search in a category : Everything else / Bulk Search in a category : hplc, factors, columns, performance |
Saturday, June 27, 2015
The 3S series high-pressure reciprocating triplex pump is mainly used in oil exploration and refinery, petrochemical, food, pharmaceutical and other light industries whereas transportation of oil, sewage, chemical and water under pressure is required. Location : ROOM 26, 5/F, THRIVING...
Maxfully International Equipment Ltd.
- 852 - TSUEN WAN
- +85 2 31 63 23 45
Saturday, July 12, 2014
Quantity : 5000 - Price : 1,50 euros
Bonjour, Vends maillots de bain filles licence High School Musical. Taille: 116-122-128-134-140-146-152-158-164-170. Prix 1,50 euros par 500 pièces. Mail. nekortex@gmail.com Mobile. 06.52.83.92.08 Mr Ben. Location : 18 rue d'albert, 95140 Garges les gonesses, Contact : Ben Mr...
SARL DTR
- 95140 - Garges les gonesses
- 06 52 83 92 08
Friday, August 12, 2011
Quantity : 1000000CAR - Price : negotiable
WE ARE REPUTED DEALERS OF GEMSTONES AND DIAMONDS.WE HAVE ALL TYPES OF GEM THAT IS RANGING FROM AQUAMARINE TO PEARL GEMS.WE SUPPLY AT AFFORDABLE PRICES AND OUR GEMS ARE OF HIGH QUALITY AND MEETS UP TO 99% PURITY.IF INTERESTED IN BUYING YOUR GEMSTONES DO NOT HESITATE TO CONTACT USLocation ...
ets.mankaa
- dunois
- - yaounde
- +23 7 96 18 29 09




