Factors Affecting the Performance of C18 HPLC Columns
Physical properties of the chromatographic column could affect the performance of C18 HPLC columns.
Performance factors of C18 HPLC columns:
Silica gel purity
Class A silica gel has high residual silica hydroxyl with a negative charge and high metal content on the acidic surface, which leads to trailing of alkaline compounds.
Due to the low metal content and high silica hydroxyl pKa of class B silica gel, tail dragging of alkaline compounds does not occur.
Performance factors of C18 HPLC columns:
Column size
Short column – short running time, low column pressure
Long column – high resolution, long running time
Narrow diameter column – detector has high sensitivity
Performance factors of C18 HPLC columns:
Particle shape and size
When the mobile phase with high viscosity is used, the spherical particles can reduce the column pressure and prolong the life of the column. Smaller particles have higher column efficiency but can cause column pressure to be too high.
Performance factors of C18 HPLC columns:
Surface area and aperture
The high surface area has strong retention capacity, column capacity and separation degree for separating multi-component samples. Low surface area fillers usually reach equilibrium quickly and are particularly important for gradient leaching.
Contact : Bruce Lu, +86 29 89284429
Good deal: buying from seller
We invite you to read our terms of use. You can also visit our FAQ section and see our information section on the risks associated with counterfeiting.
|
This page is about importers and exporters of Factors Affecting the Performance of C18 HPLC Columns Search in a category : Everything else / Bulk Search in a category : hplc, performance, factors, columns |
Sunday, June 03, 2007
Quantity : Mini 100 à - Price : sur dde
Ipods audios, videos mp3,mp4 1Gb,2Gb et + Ipods Personnalisable ( votre logo ) Packaging sur mesure Garantie Fiabilité Serieux Fabrication sur mesure directe de Chine Control Qualité Français Location : 25 grand rue, 13013 marseille, Contact : Stephane Papini, 06 20 56 39 24
kandk
- kandkimport
- 13013 - marseille
- 06 20 56 39 24
- +33 6 20 56 39 24
Sunday, January 03, 2010
Quantity : Régulier
Nous cherchons un partenariat sur le long terme avec des plateformes de SAV pouvant nous fournir en éléctroménager, meuble, ordinateur, produit audio .... de retour client. Annonce sérieuse .Location : 19, avenue Tolosane, 31520 RAMONVILLE, Contact : Fabrice Gardelle,
Friday, August 27, 2010
Quantity : by contain - Price : various
We export various high quality Japanese food and beverages, from frozen vegetables, canned food, to gree tea, coffee, and sake. Please visit our website and we are very happy that we can offer various business chances to you.Location : 1-6-8, Koyocho-naka, Higashinada-ku,, 658-0032 Kobe...
Crystal Group Co., Ltd.
- junkojat
- 658-0032 - Kobe, Hyogo
- +81 7 88 42 92 20
- 81 90 8750 1804




