After Getting The Sample, How To Choose Flash Column?
Column chromatography is found important in the labs due to its factors of easy packing procedure, low pressure of operation and low costs as well. Depend on the way that the solvent flows down the column, we can find two different kinds of column chromatography: one is gravity column chromatography when the solvent flows down the column by gravity, the other is flash column chromatography when the solvent is pushed down the column by positive air pressure.
In traditional column chromatography, we place a sample that is going to be purified or separated on the top of the column which contains silica gel or other solid support. After the column is filled with the solvent which runs through the solid support by gravity, the various components of the solvent will go through the column at a different speed. And we can collect them separately as we find them at the bottom of the column.
Compared with gravity column chromatography, flash column chromatography is basically an air pressure driven hybrid of medium pressure which can speed up the flow of solvent and save the time of sample purifying in flash chromatography. Usually, it takes less than 10-15 minutes to make the column and run the separation.
After Getting The Sample, How To Choose Flash Column?
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