HPLC - 411 ads

Wednesday, April 01, 2020

The properties of the reversed-phase filler are determined by the matrix and the substrate. the particle shape, size, pore size and pore size distribution of the matrix, specific surface area, residual silicon hydroxyl concentration, and the content of impurity ions will all have...

Wednesday, March 25, 2020

Character of syringe filter 1. The PTFE membrane of the syringe filter is completely made of natural and permanent hydrophobic PTFE material. Even under a very low pressure difference, it can ensure that moist air or other gases can pass without hindrance, while the aqueous solution...

Tuesday, March 24, 2020

Remove the column outlet end If the pressure does not drop, the HPLC column is blocked. Treatment: if the buffer salt is blocked, flush 95% water to normal pressure. If some strongly retained substances cause blockage, the mobile phase stronger than the current one should be used...

Monday, March 23, 2020

Advantages of flash column FLASH chromatography is used for preparation and has the following advantages: 1. Fast speed, generally 1/8 of the separation time of open column chromatography, saving time, slow sample change and decomposition 2. Large preparation volume, at least as compared...

Wednesday, March 18, 2020

Widely used to remove particles from a liquid sample and avoid damage to equipment at the same time, syringe filter is affordable tool in the labs, which the scientists can use for small-volume filtration. You can find two different types of filters, one is with which you can recover your sample...

Tuesday, March 17, 2020

Cleaning and storage of HPLC column Have a good understanding of the sample made, and wash it with the mobile phase that has the strongest eluting capacity for the sample. For silica gel HPLC column, first wash away the polar impurities with methanol, then use dry dichloromethane...

Tuesday, March 17, 2020

How to understand the chromatographic separation? Put it in short, the more similar the separated substance to the stationary phase, the longer the residence time on it. In this way, different substances in a complex mixed solution can be separated according to time, and the separated...

Thursday, March 12, 2020

What areSyringe filter syringe filters used for? syringe filter, also known as needle filter, cylinder filter, cylinder filter membrane filter, etc., is used for sample prefiltration, clarification of the removal of particles, liquids and gases, etc. What are the advantages of using...

Tuesday, March 10, 2020

Types of HAWACH HPLC Column There are 2 kinds of HAWACH HPLC columns: Xcheoma series: with many types of fixed phase; with complete varieties and high quality. C18-Aqueous; C18-universal;C18-low pH; C18-Alkaline; C8-universal; C8-Low pH; C8-Fluorine; C4; C30; C1;Phenyl; PAH; etc Echroma series...

Tuesday, January 21, 2020

Syringe filter is separation equipment which can be combined with a injector to filter out the impurities from the sample of chemically turbid solution. Syringe filter generally consists of a plastic housing and a membrane. The body of syringe filter (plastic housing) is usually made...

Monday, January 20, 2020

1. Why does the retention time sometimes drift and sometimes change rapidly when analyzed by HPLC? How to solve it? About drift ① The temperature control is not good. The solution is to use a constant temperature device to keep the HPLC column temperature constant. ② The mobile phase...

Friday, January 17, 2020

Vacuum filtration system is a kind of vacuum filtration device, which ingeniously combines all the components needed for Vacuum filtration on one body, saving space and simple operation. It is widely used in laboratories, including various microbial tests in food, medicine, beverage...

Tuesday, January 14, 2020

The sample shall be dissolved as much as possible in solvents that are mutually soluble with the mobile phase. Except for special designation, the resolution of the sample HPLC column may be reduced if it is dissolved using a strong solvent. Besides, the sample solution shall be pre-filtered...

Tuesday, January 07, 2020

Hawach HPLC column is composed of a column tube, pressure cap, clamping sleeve (sealing ring), the screen plate (filter), joint, screw and so on. The column tube is made of stainless steel. When the pressure is not more than 70 kg/cm2, thick-wall glass or quartz tube can also be used...

Friday, January 03, 2020

Operation of HPLC Columns In daily separation analysis, it is significant to properly use column, or the service life of the column efficiency of it can be directly affected. In the course of column loading, unloading or replacement, strong mechanical vibration should be prevented...

Friday, December 13, 2019

High-perfor mance liquid chromatography (HPLC) was developed on the basis of classical liquid chromatography and introduced gas chromatography theory in the late 1960s. It differs from classical liquid chromatography in that the filler particles are small and uniform, and the small...

Wednesday, December 11, 2019

The HPLC reversed-phase column is made of high-purity and high-strength silica gel by using advanced bonding technology. It has a high surface bonding rate and good peak shape for alkaline and acidic compounds. With a high column capacity and long service life, it is the one for the...

Wednesday, December 11, 2019

Syringe filters are used in a wide range of laboratories to provide fast and efficient water and organic solution filtration solutions. It is made from a variety of membrane filters containing polypropylene housings, and special injection molding processes increase burst pressure...

Wednesday, December 11, 2019

Flow Process of HPLC column First, the solvent in the storage bottle is pumped into the chromatographic system by pump and then output, and then introduced into the sampler after flow and pressure measurement. Second, object to be tested is injected by the sampler, passes through...

Tuesday, December 10, 2019

The protective HPLC columns influence the separation result. First of all, the protective column has a good positive effect on the experiments. Even if the stationary phase is chosen incorrectly, the protective column can also be effective for separation. The protective column is...

Saturday, October 12, 2019

1.Usage HAWACH syringe filter is used with syringes to filter chemical turbid solution samples, most commonly used in chemical HPLC-MS/GC-MS analysis of liquid and air dedusting, sterilization filtration, biological sample preparation, tissue culture media, microbial media, buffer solutions, etc...

Saturday, October 12, 2019

1. Leakage at joint Generally, it can be solved by tightening, cleaning and replacement. 2. Pump leakage Check whether the one-way valve and joint are loose, if loose and tightened (not too tight), check whether the seal of mixer and pump is damaged; 3. Leakage of injection valve...

Thursday, October 10, 2019

The essence of the chromatographic process is the process of distributing the equilibrium of the molecules of the substance to be separated between the stationary phase and the mobile phase. The distribution of the different substances between the two phases will be different, which...

Wednesday, October 09, 2019

Hawach Scientific co., LTD. is a professional technology company integrating analytical chemical product development, manufacturing, sales, and service, Since its establishment, the company has maintained a high speed and steady pace of development, along with scientific and technological...

Wednesday, October 09, 2019

Generally speaking，with the increase of temperature, the viscosity of the mobile phase decreases. The situation will lead to an increase in the transfer rate of the substances. Thus the chromatographic separation effect will be better than before. Although the separation effect has been improved...

Tuesday, October 08, 2019

The essence of the chromatographic process is the process of distributing the equilibrium of the molecules of the substance to be separated between the stationary phase and the mobile phase. The distribution of the different substances between the two phases will be different, which...

Monday, September 30, 2019

HPLC columns are widely used in many fields, whose column efficiency, capacity, permeability characteristics, and service life are very important. After using for a period of time, there will be changes such as column pressure increase, column efficiency decrease, etc. If no measures are taken...

Sunday, September 29, 2019

There are many types of chromatographic methods, and different classification methods can be used from different perspectives. Classification from the state of two phases. In the chromatography, the mobile phase may be a gas or a liquid, and thus can be classified into gas chromatography...

Saturday, September 28, 2019

Depending on the packing of the HPLC column. The following column types are available on the market: 1. Silica gel HPLC column: Silica gel is the earliest chromatographic column packing used in liquid chromatography. It has a wide range of applications. However, it can only be used...

Wednesday, September 25, 2019

Knowledge of QuEChERS Quick, Easy, Cheap, Effective, Rugged, Safe are generally abbreviated to QuEChERS. It is a sample pretreatment method for agricultural product detection. It is used to absorb and remove impurity, and the operational principle is similar to high-performance liquid...