Property And Principle Of Reversed Phase C4 HPLC Column

The properties of the reversed-phase filler are determined by the matrix and the substrate. the particle shape, size, pore size and pore size distribution of the matrix, specific surface area, residual silicon hydroxyl concentration, and the content of impurity ions will all have an effect on the chromatographic behavior of the reversed phase filler. And the type of the ligand, the way of connection, the density of the ligand, etc., determine the retention behavior of the solute. Additionally, reversed phase C4 HPLC column is a chromatographic model based on the hydrophobic effect between the surface of solute, polar mobile phase and nonpolar stationary phase. The structure of any organic molecule has a nonpolar hydrophobic part.

Characteristic of Reversed Phase C4 HPLC Column
Hawach uses highly controllable monolayer formation and tail sealing techniques to produce reversed phase C4 HPLC column. It features high inter-column reproducibility, high selectivity, and separation efficiency. Moreover, it is suitable for the separation of acidic, neutral and alkaline compounds, as well as many drugs and peptides, etc. the mobile phase of an organic solvent or organic solvent/water system is recommended.

Property And Principle Of Reversed Phase C4 HPLC Column