Medical
Triiodothyronine (T3) |
Manufacturer |
SEKBIO |
Parameter |
TT3 |
Linearity |
0.3-10 nmol/L |
Detection limit |
0.3 nmol/L |
Benchmark product |
Roche |
Test principle and procedure |
TT3: Add 30 μl of sample + 30 μl of Rd, incubation for 10 minutes without cleaning, 30 μl of Ra + 30 μl of Rc and incubation for 5 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Precision
Sample |
RLU |
Concentration |
Sample |
RLU |
Concentration |
0.5 |
270,946 |
0.47 |
5 |
83,336 |
4.10 |
276,899 |
0.45 |
79,406 |
4.42 |
||
278,536 |
0.44 |
82,669 |
4.16 |
||
259,590 |
0.52 |
84,427 |
4.02 |
||
269,690 |
0.48 |
81,363 |
4.26 |
||
270,230 |
0.47 |
79,263 |
4.44 |
||
257,837 |
0.53 |
80,565 |
4.33 |
||
270,880 |
0.47 |
77,389 |
4.60 |
||
279,936 |
0.44 |
81,233 |
4.27 |
||
271,277 |
0.47 |
79,113 |
4.45 |
||
AVE |
270582.10 |
0.47 |
AVE |
80876.40 |
4.30 |
SD |
7269.04 |
0.03 |
SD |
2169.67 |
0.18 |
CV |
2.7% |
6.1% |
CV |
2.7% |
4.2% |
Limit of detection
Sample |
RLU |
Concentration |
||
Ag Diluent |
980,305 |
1,022,449 |
0 |
0 |
986,963 |
1,001,873 |
0 |
0 |
|
984,209 |
1,003,978 |
0 |
0 |
|
1,026,252 |
1,000,042 |
0 |
0 |
|
1,018,574 |
961,333 |
0 |
0 |
|
974,731 |
982,278 |
0 |
0 |
|
1,022,323 |
969,066 |
0 |
0 |
|
1,071,730 |
968,489 |
0 |
0 |
|
1,043,724 |
1,028,076 |
0 |
0 |
|
1,014,595 |
1,014,797 |
0 |
0 |
|
AVE |
1003789.35 |
0.00 |
||
SD |
28331.30 |
0.00 |
||
AVE+2*SD |
0 |
Linear
Sample(nmol/L) |
Dilution ratio |
Concentration value |
Average |
||
10.13 |
1.0 |
10.41 |
10.67 |
10.13 |
10.41 |
8.14 |
0.8 |
8.02 |
7.86 |
8.03 |
7.97 |
6.15 |
0.6 |
5.96 |
5.90 |
5.94 |
5.94 |
4.16 |
0.4 |
3.67 |
3.72 |
3.66 |
3.68 |
2.16 |
0.2 |
1.79 |
1.75 |
1.70 |
1.75 |
0.17 |
0.0 |
0.17 |
0.18 |
0.16 |
0.17 |
Method Comparison
Reagents Stability
Reagent |
Sample(nmol/L) |
Average |
Deviation |
2-8℃ |
9.18 |
55,377 |
|
4.71 |
81,863 |
||
0.43 |
340,779 |
||
Antigen diluent |
666,737 |
||
random sample |
200,281 |
||
calf serum |
453,520 |
||
37℃ 1d |
9.18 |
53,767 |
-2.91% |
4.71 |
83,258 |
1.70% |
|
0.43 |
339,943 |
-0.25% |
|
Antigen diluent |
664,104 |
-0.39% |
|
random sample |
188,472 |
-5.90% |
|
calf serum |
459,529 |
1.32% |
|
37℃ 3d |
9.18 |
51,653 |
-6.72% |
4.71 |
82,714 |
1.04% |
|
0.43 |
360,888 |
5.90% |
|
Antigen diluent |
645,376 |
-3.20% |
|
random sample |
198,372 |
-0.95% |
|
calf serum |
480,304 |
5.91% |
|
37℃ 7d |
9.18 |
49,977 |
-9.75% |
4.71 |
78,711 |
-3.85% |
|
0.43 |
366,369 |
7.51% |
|
Antigen diluent |
629,915 |
-5.52% |
|
random sample |
189,952 |
-5.16% |
|
calf serum |
486,154 |
7.20% |
Calibrator Stability
Reagent |
Sample(nmol/L) |
Average |
Deviation |
2-8℃ |
6.70 |
43,902 |
|
3.70 |
74,473 |
||
0.32 |
310,740 |
||
37℃ 1d |
6.70 |
43,667 |
|
3.70 |
76,993 |
-0.53% |
|
0.32 |
307,831 |
3.38% |
|
37℃ 3d |
6.70 |
43,303 |
-0.94% |
3.70 |
75,963 |
-1.36% |
|
0.32 |
306,706 |
2.00% |
|
37℃ 7d
|
6.70 |
44,214 |
-1.30% |
3.70 |
72,454 |
0.71% |
|
0.32 |
305,293 |
-2.71% |
As a professional tumor marker factory, SEKBIO has high-quality tumor marker for sale. Welcome to contact us if needed.
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