Bonded-Phase Chromatography

Bonded-phase chromatography is a liquid-liquid chromatography method in which a stationary phase is covalently bound to a carrier particle, which overcomes the mechanical shock in the distribution chromatography, the constant loss of the stationary phase and the gradual change of the nature of the column.

Here we have a Quickstart for filler and model selection based on bonded-phase.

For highly polar samples, use C18, C8, amino, cyano bonded phase silica gel fillers or butyl, octyl, phenyl bonded phase agarose and dextran fillers, or polymer fillers, reversed Separation.

For medium and low polarity samples, normal phase separation or hydrophilic chromatography separation using amino group-containing, diol-based, and surface-modified silica-bonded silica gel fillers.

For high molecular weight samples, size exclusion (gel filtration) is performed with agarose and dextran or silica gel packing.

Specific structural samples, optional filler matrices including agarose and dextran containing metal polymerization, activation coupling, small ligand affinity, antibody affinity, pigment affinity bonding phase, and choral groups, crowns Polymer-bonded phase polymer packing, separated by affinity chromatography.

The ionizable sample, the ion exchange method and the filler matrix were separated and purified by agarose and dextran containing an SP.Q.CM.DEAE bonded phase, silica gel, and a polymer epoxy resin by ion exchange.